Facile and Effective Pretreatment Using Stop and Go Extraction Tips for LC-MS/MS Analysis of Trace Amounts of DNA Adducts

The preparation and application of a stop and go extraction tip (StageTip) used for pre-purification of sample solutions for LC-MS/MS analysis of DNA adducts was simplified and improved to increase throughput while maintaining high adduct selectivity. It was demonstrated that the StageTip composed o...

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Veröffentlicht in:	Analytical Sciences 2014/04/10, Vol.30(4), pp.519-522
Hauptverfasser:	MURAKAMI, Hiroya, KAWAMURA, Rieko, SAKAKIBARA, Takayoshi, ESAKA, Yukihiro, ISHIHAMA, Yasushi, UNO, Bunji
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytic Sample Preparation Methods - instrumentation Analytic Sample Preparation Methods - methods Analytical Chemistry Chemical Fractionation Chemistry Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Deoxyribonucleosides - chemistry DNA adduct DNA Adducts - analysis High-Throughput Screening Assays LC-MS/MS analysis poly(styrene-divinylbenzene) copolymer Polystyrenes - chemistry Sensitivity and Specificity Solutions stop and go extraction tip Tandem Mass Spectrometry - instrumentation Tandem Mass Spectrometry - methods
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Zusammenfassung:	The preparation and application of a stop and go extraction tip (StageTip) used for pre-purification of sample solutions for LC-MS/MS analysis of DNA adducts was simplified and improved to increase throughput while maintaining high adduct selectivity. It was demonstrated that the StageTip composed of two sheets of a poly(styrene-divinylbenzene) copolymer disk could be easily prepared and proved useful for selective extraction of trace amounts of DNA adducts from a sample solution containing a great quantity of normal deoxynucleosides.
ISSN:	0910-6340 1348-2246
DOI:	10.2116/analsci.30.519