Interpreting HIV ELISA reactivity: Alternatives to western blot

Interpreting HIV ELISA reactivity: Alternatives to western blot

Three types of tests have been used to confirm the presence of antibody to HIV identified by the ELISA screening assay: (1) Western blot, (2) radioimmunoprecipitation (RIP), and (3) immunofluorescence. This paper discusses the technical differences in these three assays, factors affecting their rela...

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Veröffentlicht in:Journal of clinical laboratory analysis 1988, Vol.2 (3), p.174-181
Hauptverfasser: Britz, Judith A., Rolon, Noel, Hill, Todd, Page, Eileen, Geltosky, Jack
Format: Artikel
Sprache:eng
Schlagworte:
HIV confirmation
HIV ELISA
human immunodeficiency virus
Western blot
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Zusammenfassung:Three types of tests have been used to confirm the presence of antibody to HIV identified by the ELISA screening assay: (1) Western blot, (2) radioimmunoprecipitation (RIP), and (3) immunofluorescence. This paper discusses the technical differences in these three assays, factors affecting their relative sensitivity and specificity, and their interpretation. A table shows major features of each assay. In light of their collective experience, alternative approaches are discussed.
ISSN:0887-8013
1098-2825
DOI:10.1002/jcla.1860020310