Recombinant goose-type lysozyme in channel catfish: lysozyme activity and efficacy as plasmid DNA immunostimulant against Aeromonas hydrophila infection

The objectives of this study were: 1) to investigate whether recombinant channel catfish lysozyme-g (CC-Lys-g) produced in Escherichia coli expression system possesses any lysozyme activity; and 2) to evaluate whether channel catfish lysozyme-g plasmid DNA could be used as an immunostimulant to prot...

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Veröffentlicht in:	Fish & shellfish immunology 2013-10, Vol.35 (4), p.1309-1319
Hauptverfasser:	Pridgeon, Julia W, Klesius, Phillip H, Dominowski, Paul J, Yancey, Robert J, Kievit, Michele S
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinomycetales Infections - genetics Actinomycetales Infections - prevention & control Actinomycetales Infections - veterinary Adjuvants, Immunologic - chemistry Adjuvants, Immunologic - genetics Adjuvants, Immunologic - metabolism Aeromonas hydrophila Aeromonas hydrophila - immunology Aeromonas hydrophila - pathogenicity Aeromonas hydrophila - physiology Amino Acid Sequence Animals bacterial infections Cloning, Molecular DNA, Complementary - genetics DNA, Complementary - metabolism Escherichia coli Escherichia coli - genetics fish fish diseases Fish Diseases - genetics Fish Diseases - prevention & control Fish Proteins - chemistry Fish Proteins - genetics Fish Proteins - metabolism gene expression gills Gram-Negative Bacterial Infections - genetics Gram-Negative Bacterial Infections - prevention & control Gram-Negative Bacterial Infections - veterinary Ictaluridae - genetics Ictaluridae - immunology Ictaluridae - metabolism Ictalurus punctatus immunization lysozyme macrophages Micrococcus - immunology Micrococcus - physiology Micrococcus luteus Molecular Sequence Data Muramidase - chemistry Muramidase - genetics Muramidase - metabolism nitric oxide Phylogeny plasmids Plasmids - genetics reactive oxygen species Real-Time Polymerase Chain Reaction - veterinary recombinant proteins Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment - veterinary transfection
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Zusammenfassung:	The objectives of this study were: 1) to investigate whether recombinant channel catfish lysozyme-g (CC-Lys-g) produced in Escherichia coli expression system possesses any lysozyme activity; and 2) to evaluate whether channel catfish lysozyme-g plasmid DNA could be used as an immunostimulant to protect channel catfish against Aeromonas hydrophila infection. Recombinant CC-Lys-g produced in E. coli expression system exhibited significant (P
ISSN:	1050-4648 1095-9947
DOI:	10.1016/j.fsi.2013.08.015