G-Banding of the Chromosomes of Apogon maculatus and A. pseudomaculatus (Perciformes: Apogonidae)

In a continuation of studies on the cytogenetics of members of the Apogonidae two further methods of G-banding were applied to chromosome preparations of gill epithelial tissue of Apagon maculatus and A. pseudomaculatus . A modification of the trypsin G-binding procedure of Arrighi and Hsu super(1) was applied to the chromosomes of A. pseudomaculatus) collected from the Florida Keys. The method outlined in Rivlin et al. involves colchicine treatment (0.05 mg colchicine per gram of body weight), hypotonic pretreatment (0.4% KCl) of the gill arches, and absolute methanol-glacial acetic acid (3:1) fixation, dabbing the gill filaments onto water-coated precleaned glass slides followed by rapid air-drying.