IN VITRO ANTIBACTERIAL, ANTIOXIDANT, HAEMOLYTIC, THROMBOLYTIC ACTIVITIES AND PHYTOCHEMICAL ANALYSIS OF SIMAROUBA GLAUCA LEAVES EXTRACTS

This paper aims to study the leaves of Simarouba glauca for their antibacterial, antioxidant, haemolytic, thrombolytic activities and to perform phytochemical evaluation. The three extracts (chloroform, methanol, ethyl acetate) of Simarouba glauca were screened for antibacterial activity against five pathogenic microorganisms by well diffusion method. In vitro antioxidant activity of extract was studied using H2O2 radical scavenging assay. The haemolytic activity was determined using agar diffusion techniques on blood agar plate, thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis. Among the different extracts tested, the methanol extract of leaves showed significant antimicrobial activities. The most susceptible micro-organisms were found to be gram negative bacteria (Stenotrophomonas maltophilia, Citrobacter), gram positive bacteria (Enterococcus faecalis). H2O2 scavenging activity of Simarouba glauca was found to increase with increasing concentration of the extract. IC sub( 50) values of H2O2 scavenging activity was 6.72 plus or minus 0.1 mu g/mL which was found in chloroform extract. This study shows that all the extract of leaves of Simarouba glauca has bioactivity.