IDENTIFICATION, MOLECULAR CHARACTERIZATION AND EVALUATION OF SOME RUMINAL BACTERIA THAT CATALYZE AND DEGRADE PLANT CELLULOSIC MATERIALS

In the last few years, there was a growing economic awareness about plant wastes and use it in compost production or in feeding of farm animals, some of these wastes are useless and cause a lot of problems in Egyptian environment. This study was designed to isolate, identify and evaluate some ruminal bacterial isolates to facilitate the in vitro degradation of certain local agro-industrial wastes (rice straw and corn stalks). Two bacterial isolates were isolated from rumen fluid of some Egyptian cattle and identified as Fibrobacter succinogenes and Ruminococcus albus depending on morphological, biochemical identification and molecular characterization using 16S rRNA genes. Two degenerate primers were used to detect about 1.6 kb PCR fragment, a partial sequences (709 and 401) bp were sequenced for Fibrobacter succinogenes and Ruminococcus albus respectively. The highest similarity was detected for both isolates, which were 57% with Streptomyces sp. for Fibrobacter succinogenes and 77% with Enterobacter sp. for Ruminococcus albus.