Identification of Several Mycobacterium Species by using Amplified 16S Ribosomal DNA

In order to diagnose MOTT species M. smegmatisand M. kansasii along with M. tuberculosis, species specificprimers were designed using PRIMER-BLAST which amplifiedparticular 16S rRNA genes having variable amplicons sizes. Allprimer sets were found to be specific for their target 16S regionsof mycobacterium species. Easy and fast DNA isolation protocoldeveloped that could be implemented directly for PCR reactionof the samples. By involving standardized PCR conditions withdefined primers and genome combinations, we havesuccessfully reported the 16S rRNA gene based PCR detectionof several mycobacterium species which has highlighted theMOTT detection capabilities.