Age and isolation influence steroids release and chemical signaling in male mice

•Age influences the release of stress hormone corticosterone and androgens.•Social interactions shape both neuroendocrine functions and behavior.•Young and adult male mice kept either isolated or in group were studied.•Isolation stimulates androgen secretion and excretion of some pheromones. Young mice show higher plasma corticosterone.•Apparently, adult mice adapt to long term isolation, with less severe consequences. Social interactions in mice involve olfactory signals, which convey information about the emitter. In turn, the mouse social and physiological status may modify the release of chemical cues. In this study, the influences of age and social isolation on the endocrine response and the release of chemical signals were investigated in male CD1 mice, allocated into four groups: Young Isolated (from weaning till 60days; N=6), Adult Isolated (till 180days; N=6), Young Grouped (6 mice/cage; till 60days; N=18), Adult Grouped (6 mice/cage; till 180days; N=18). Mice were transferred in a clean cage to observe the micturition pattern and then sacrificed. Body and organs weights, serum testosterone, dehydroepiandrosterone, corticosterone and the ratio Major Urinary Protein/creatinine were measured. Urinary volatile molecules potentially involved in pheromonal communication were identified. Androgen secretion was greater in isolated mice (P