Development of an indirect competitive assay-based aptasensor for highly sensitive detection of tetracycline residue in honey

Tetracycline (TC) is widely used for prevention and control of animal diseases for its broad spectrum antimicrobial activity and low cost, but its abuse can seriously affect human health and may result in trade loss. Thus there is an imperative need to develop high-performing analytical technique for TC detection. In this study, we developed a biosensor based on an indirect competitive enzyme-linked aptamer assay (ic-ELAA). A 76mer single-stranded DNA (ssDNA) aptamer, selected by Systematic Evolution of Ligands by Exponential Enrichment (SELEX), was applied for the recognition and detection of TC in honey. The limit of detection was 9.6×10−3ng/mL with a linear working range from 0.01 to 100ng/mL toward TC in honey, and a mean recovery rate of 93.23% in TC-spiked honey was obtained. This aptasensor can be applied to detect TC residue in food with high sensitivity and simplicity, and it is prospective to develop useful ELAA Kits for TC determination in food. •An aptasensor was developed to detect tetracycline in honey with simplicity.•Detailed stepwise optimization for working conditions was carried out.•The biosensor presented superior sensitivity and a relatively wide linear range.•The biosensor might be utilized for high-throughput TC detection.