In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study
Background Saline‐adenine‐glucose‐mannitol (SAGM) and a variant solution, AS‐1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired...
Gespeichert in:
| Veröffentlicht in: | Transfusion (Philadelphia, Pa.) Pa.), 2014-03, Vol.54 (3), p.560-568 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 568 |
|---|---|
| container_issue | 3 |
| container_start_page | 560 |
| container_title | Transfusion (Philadelphia, Pa.) |
| container_volume | 54 |
| creator | Sparrow, Rosemary L. Sran, Amrita Healey, Geraldine Veale, Margaret F. Norris, Philip J. |
| description | Background
Saline‐adenine‐glucose‐mannitol (SAGM) and a variant solution, AS‐1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM RBCs and AS‐1 RBCs was conducted to identify membrane changes, including microparticle (MP) quantitation and in vitro RBC–endothelial cell (EC) interaction.
Study Design and Methods
Two whole blood packs were pooled and split and RBCs were prepared (n = 6 pairs). One pack was suspended in SAGM and one in AS‐1. Samples were collected during 42 days of refrigerated storage. RBC shape and size and glycophorin A (GPA)+ and phosphatidylserine (PS)+ MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine variables (pH, hemolysis) were also measured.
Results
Compared to SAGM RBCs, AS‐1 RBCs had lower hemolysis (p |
| doi_str_mv | 10.1111/trf.12344 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1507189337</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3242132281</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3714-755e3aa289be3caf74a880fd9ae20986260953b8ed30ab7ec240fa910fe0f53</originalsourceid><addsrcrecordid>eNp1kc9u1DAQxiMEokvhwAsgSwgJDmntOI5jblWhf6QKJLoSR2uSjIuL117sZMu-EM-Jw26LhIQvY41_882Mv6J4yegRy-d4jOaIVbyuHxULJrgsK6XE42JBac1Kxnh1UDxL6ZZSWinKnhYHFW8b1dBqUfy69GRjxxjICiFNERMJJt9XXQSPpP8G_ibnIm4QHBmsMRjR9znV4XiH6PPTQDoXwkB6dC6RNIY5ZT1J4KzHEgb0c7xxUx8Slivw3o7BEfADObkuGYFhsKPdIEnBTaMNPr0nQNZgZ6E0TsP2efHEgEv4Yh8Pi-uzj8vTi_Lq8_nl6clV2XPJ6lIKgRygalWHvAcja2hbagYFWFHVNlVDleBdiwOn0Ensq5oaUIwapEbww-LtTnUdw48J06hXNs1b5a8IU9JMUMlaxbnM6Ot_0NswRZ9nmylRt5VseKbe7ag-hpQiGr2OdgVxqxnVs3U6W6f_WJfZV3vFqVvh8EDee5WBN3sAUg_OZIN6m_5yLVei4fMWxzvuzjrc_r-jXn45u29d7ipsGvHnQwXE77qRXAr99dO5vhD8Q8OXtZb8N9NXwKg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1505482763</pqid></control><display><type>article</type><title>In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Sparrow, Rosemary L. ; Sran, Amrita ; Healey, Geraldine ; Veale, Margaret F. ; Norris, Philip J.</creator><creatorcontrib>Sparrow, Rosemary L. ; Sran, Amrita ; Healey, Geraldine ; Veale, Margaret F. ; Norris, Philip J.</creatorcontrib><description>Background
Saline‐adenine‐glucose‐mannitol (SAGM) and a variant solution, AS‐1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM RBCs and AS‐1 RBCs was conducted to identify membrane changes, including microparticle (MP) quantitation and in vitro RBC–endothelial cell (EC) interaction.
Study Design and Methods
Two whole blood packs were pooled and split and RBCs were prepared (n = 6 pairs). One pack was suspended in SAGM and one in AS‐1. Samples were collected during 42 days of refrigerated storage. RBC shape and size and glycophorin A (GPA)+ and phosphatidylserine (PS)+ MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine variables (pH, hemolysis) were also measured.
Results
Compared to SAGM RBCs, AS‐1 RBCs had lower hemolysis (p < 0.04), lower GPA+ MPs (p < 0.03), and lower PS+ MPs (p < 0.03) from Day 14 onward. AS‐1 RBCs had higher (p < 0.02) side scatter from Day 28 onward compared to SAGM RBCs. SAGM RBCs were more adherent to ECs on Day 28 of storage compared to AS‐1 RBCs (p = 0.04), but reversed on Day 42 (p = 0.02).
Conclusion
SAGM RBCs lose more membrane during storage. SAGM RBCs had increased adherence to ECs on Day 28 of storage, while AS‐1 RBCs were more adherent on Day 42. The effect of these differences on the function and survival of SAGM RBCs and AS‐1 RBCs after transfusion remains to be determined.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1111/trf.12344</identifier><identifier>PMID: 23869602</identifier><identifier>CODEN: TRANAT</identifier><language>eng</language><publisher>Hoboken, NJ: Blackwell Publishing Ltd</publisher><subject>Adenine - pharmacology ; Adult ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Blood ; Blood Preservation - methods ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Erythrocytes ; Erythrocytes - drug effects ; Flow Cytometry ; Glucose - pharmacology ; Humans ; Mannitol - pharmacology ; Medical sciences ; Middle Aged ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Young Adult</subject><ispartof>Transfusion (Philadelphia, Pa.), 2014-03, Vol.54 (3), p.560-568</ispartof><rights>2013 Australian Red Cross. Transfusion © 2013 American Association of Blood Banks</rights><rights>2015 INIST-CNRS</rights><rights>2013 Australian Red Cross. Transfusion © 2013 American Association of Blood Banks.</rights><rights>2014 AABB</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3714-755e3aa289be3caf74a880fd9ae20986260953b8ed30ab7ec240fa910fe0f53</citedby><cites>FETCH-LOGICAL-c3714-755e3aa289be3caf74a880fd9ae20986260953b8ed30ab7ec240fa910fe0f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftrf.12344$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftrf.12344$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28395635$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23869602$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sparrow, Rosemary L.</creatorcontrib><creatorcontrib>Sran, Amrita</creatorcontrib><creatorcontrib>Healey, Geraldine</creatorcontrib><creatorcontrib>Veale, Margaret F.</creatorcontrib><creatorcontrib>Norris, Philip J.</creatorcontrib><title>In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study</title><title>Transfusion (Philadelphia, Pa.)</title><addtitle>Transfusion</addtitle><description>Background
Saline‐adenine‐glucose‐mannitol (SAGM) and a variant solution, AS‐1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM RBCs and AS‐1 RBCs was conducted to identify membrane changes, including microparticle (MP) quantitation and in vitro RBC–endothelial cell (EC) interaction.
Study Design and Methods
Two whole blood packs were pooled and split and RBCs were prepared (n = 6 pairs). One pack was suspended in SAGM and one in AS‐1. Samples were collected during 42 days of refrigerated storage. RBC shape and size and glycophorin A (GPA)+ and phosphatidylserine (PS)+ MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine variables (pH, hemolysis) were also measured.
Results
Compared to SAGM RBCs, AS‐1 RBCs had lower hemolysis (p < 0.04), lower GPA+ MPs (p < 0.03), and lower PS+ MPs (p < 0.03) from Day 14 onward. AS‐1 RBCs had higher (p < 0.02) side scatter from Day 28 onward compared to SAGM RBCs. SAGM RBCs were more adherent to ECs on Day 28 of storage compared to AS‐1 RBCs (p = 0.04), but reversed on Day 42 (p = 0.02).
Conclusion
SAGM RBCs lose more membrane during storage. SAGM RBCs had increased adherence to ECs on Day 28 of storage, while AS‐1 RBCs were more adherent on Day 42. The effect of these differences on the function and survival of SAGM RBCs and AS‐1 RBCs after transfusion remains to be determined.</description><subject>Adenine - pharmacology</subject><subject>Adult</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Blood Preservation - methods</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Erythrocytes</subject><subject>Erythrocytes - drug effects</subject><subject>Flow Cytometry</subject><subject>Glucose - pharmacology</subject><subject>Humans</subject><subject>Mannitol - pharmacology</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><subject>Young Adult</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9u1DAQxiMEokvhwAsgSwgJDmntOI5jblWhf6QKJLoSR2uSjIuL117sZMu-EM-Jw26LhIQvY41_882Mv6J4yegRy-d4jOaIVbyuHxULJrgsK6XE42JBac1Kxnh1UDxL6ZZSWinKnhYHFW8b1dBqUfy69GRjxxjICiFNERMJJt9XXQSPpP8G_ibnIm4QHBmsMRjR9znV4XiH6PPTQDoXwkB6dC6RNIY5ZT1J4KzHEgb0c7xxUx8Slivw3o7BEfADObkuGYFhsKPdIEnBTaMNPr0nQNZgZ6E0TsP2efHEgEv4Yh8Pi-uzj8vTi_Lq8_nl6clV2XPJ6lIKgRygalWHvAcja2hbagYFWFHVNlVDleBdiwOn0Ensq5oaUIwapEbww-LtTnUdw48J06hXNs1b5a8IU9JMUMlaxbnM6Ot_0NswRZ9nmylRt5VseKbe7ag-hpQiGr2OdgVxqxnVs3U6W6f_WJfZV3vFqVvh8EDee5WBN3sAUg_OZIN6m_5yLVei4fMWxzvuzjrc_r-jXn45u29d7ipsGvHnQwXE77qRXAr99dO5vhD8Q8OXtZb8N9NXwKg</recordid><startdate>201403</startdate><enddate>201403</enddate><creator>Sparrow, Rosemary L.</creator><creator>Sran, Amrita</creator><creator>Healey, Geraldine</creator><creator>Veale, Margaret F.</creator><creator>Norris, Philip J.</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201403</creationdate><title>In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study</title><author>Sparrow, Rosemary L. ; Sran, Amrita ; Healey, Geraldine ; Veale, Margaret F. ; Norris, Philip J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3714-755e3aa289be3caf74a880fd9ae20986260953b8ed30ab7ec240fa910fe0f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adenine - pharmacology</topic><topic>Adult</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Blood Preservation - methods</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Erythrocytes</topic><topic>Erythrocytes - drug effects</topic><topic>Flow Cytometry</topic><topic>Glucose - pharmacology</topic><topic>Humans</topic><topic>Mannitol - pharmacology</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sparrow, Rosemary L.</creatorcontrib><creatorcontrib>Sran, Amrita</creatorcontrib><creatorcontrib>Healey, Geraldine</creatorcontrib><creatorcontrib>Veale, Margaret F.</creatorcontrib><creatorcontrib>Norris, Philip J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sparrow, Rosemary L.</au><au>Sran, Amrita</au><au>Healey, Geraldine</au><au>Veale, Margaret F.</au><au>Norris, Philip J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><addtitle>Transfusion</addtitle><date>2014-03</date><risdate>2014</risdate><volume>54</volume><issue>3</issue><spage>560</spage><epage>568</epage><pages>560-568</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><coden>TRANAT</coden><abstract>Background
Saline‐adenine‐glucose‐mannitol (SAGM) and a variant solution, AS‐1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM RBCs and AS‐1 RBCs was conducted to identify membrane changes, including microparticle (MP) quantitation and in vitro RBC–endothelial cell (EC) interaction.
Study Design and Methods
Two whole blood packs were pooled and split and RBCs were prepared (n = 6 pairs). One pack was suspended in SAGM and one in AS‐1. Samples were collected during 42 days of refrigerated storage. RBC shape and size and glycophorin A (GPA)+ and phosphatidylserine (PS)+ MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine variables (pH, hemolysis) were also measured.
Results
Compared to SAGM RBCs, AS‐1 RBCs had lower hemolysis (p < 0.04), lower GPA+ MPs (p < 0.03), and lower PS+ MPs (p < 0.03) from Day 14 onward. AS‐1 RBCs had higher (p < 0.02) side scatter from Day 28 onward compared to SAGM RBCs. SAGM RBCs were more adherent to ECs on Day 28 of storage compared to AS‐1 RBCs (p = 0.04), but reversed on Day 42 (p = 0.02).
Conclusion
SAGM RBCs lose more membrane during storage. SAGM RBCs had increased adherence to ECs on Day 28 of storage, while AS‐1 RBCs were more adherent on Day 42. The effect of these differences on the function and survival of SAGM RBCs and AS‐1 RBCs after transfusion remains to be determined.</abstract><cop>Hoboken, NJ</cop><pub>Blackwell Publishing Ltd</pub><pmid>23869602</pmid><doi>10.1111/trf.12344</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-1132 |
| ispartof | Transfusion (Philadelphia, Pa.), 2014-03, Vol.54 (3), p.560-568 |
| issn | 0041-1132 1537-2995 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1507189337 |
| source | MEDLINE; Wiley Journals |
| subjects | Adenine - pharmacology Adult Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Blood Blood Preservation - methods Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Erythrocytes Erythrocytes - drug effects Flow Cytometry Glucose - pharmacology Humans Mannitol - pharmacology Medical sciences Middle Aged Transfusions. Complications. Transfusion reactions. Cell and gene therapy Young Adult |
| title | In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T04%3A45%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vitro%20measures%20of%20membrane%20changes%20reveal%20differences%20between%20red%20blood%20cells%20stored%20in%20saline-adenine-glucose-mannitol%20and%20AS-1%20additive%20solutions:%20a%20paired%20study&rft.jtitle=Transfusion%20(Philadelphia,%20Pa.)&rft.au=Sparrow,%20Rosemary%20L.&rft.date=2014-03&rft.volume=54&rft.issue=3&rft.spage=560&rft.epage=568&rft.pages=560-568&rft.issn=0041-1132&rft.eissn=1537-2995&rft.coden=TRANAT&rft_id=info:doi/10.1111/trf.12344&rft_dat=%3Cproquest_cross%3E3242132281%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1505482763&rft_id=info:pmid/23869602&rfr_iscdi=true |