In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study
Background Saline‐adenine‐glucose‐mannitol (SAGM) and a variant solution, AS‐1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired...
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Veröffentlicht in: | Transfusion (Philadelphia, Pa.) Pa.), 2014-03, Vol.54 (3), p.560-568 |
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Sprache: | eng |
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Zusammenfassung: | Background
Saline‐adenine‐glucose‐mannitol (SAGM) and a variant solution, AS‐1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM RBCs and AS‐1 RBCs was conducted to identify membrane changes, including microparticle (MP) quantitation and in vitro RBC–endothelial cell (EC) interaction.
Study Design and Methods
Two whole blood packs were pooled and split and RBCs were prepared (n = 6 pairs). One pack was suspended in SAGM and one in AS‐1. Samples were collected during 42 days of refrigerated storage. RBC shape and size and glycophorin A (GPA)+ and phosphatidylserine (PS)+ MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine variables (pH, hemolysis) were also measured.
Results
Compared to SAGM RBCs, AS‐1 RBCs had lower hemolysis (p |
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ISSN: | 0041-1132 1537-2995 |
DOI: | 10.1111/trf.12344 |