Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories
The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not de...
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Veröffentlicht in: | Genetics and molecular research 2014-01, Vol.13 (1), p.716-722 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains. Additionally, eight Shigella sonnei isolates were tested and found to be indistinguishable from E. coli by MALDI-TOF MS under the test conditions. |
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ISSN: | 1676-5680 1676-5680 |
DOI: | 10.4238/2014.January.29.2 |