Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories

The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not de...

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Veröffentlicht in:Genetics and molecular research 2014-01, Vol.13 (1), p.716-722
Hauptverfasser: Dallagassa, C B, Huergo, L F, Stets, M I, Pedrosa, F O, Souza, E M, Cruz, L M, Assis, F E A, Wolf, S, Volanski, W, Picheth, G, Pigatto-Denardi, C P, Farah, S M S S, Fadel-Picheth, C M T
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Sprache:eng
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Zusammenfassung:The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains. Additionally, eight Shigella sonnei isolates were tested and found to be indistinguishable from E. coli by MALDI-TOF MS under the test conditions.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.January.29.2