Synthesis and Analytical Application of a Novel Fluorescent Hg2+ Probe 3a2, 6a2-Bis(Diethylamino)-2-((2,4-Dimethoxybenzylidene)Amino)Spiro[Iso indoline-1,9a2-Xanthene]-3-Thione

A novel probe, 3a2,6a2 - bis(diethylamino) -2- ((2,4-dimethoxybenzylidene)amino) spiro [isoindoline-1,9a2-xanthene]-3-thione (RBS), was designed and synthesized. Its structure was characterized with elemental analysis, IR spectra and 1H NMR. The probe displayed highly selective and sensitive recognition of Hg2+. Reacting with mercury ions in aqueous solution, its fluorescence intensity was enhanced significantly, while its color was changed from colorless to pink. So, a new fluorescence method of detection of Hg2+ was proposed. Its dynamic response concentration range and detection limit for Hg2+ were 5.00aAa10a9 M to 1.00aAa10a6 M detected and 1.83aAa10a9 M, respectively. Satisfying results were obtained when the probe was applied to detect spiked Hg2+ in samples.