New constituents from the dried fruit of Piper nigrum Linn., and their larvicidal potential against the Dengue vector mosquito Aedes aegypti

Three new constituents namely pipilyasine (1), pipzubedine (2) and pipyaqubine (3) were isolated from the seeds extract of Piper nigrum Linn., along with three known constituents pellitorine (4), pipericine (5) and piperine (6) following a larvicidal activity guided isolation against 4th instar larvae of Aedes aegypti L., a Dengue vector mosquito and a carrier of yellow fever. Their structures were elucidated using modern spectroscopic methods. The larvicidal activity was determined by WHO method. [Display omitted] ► Three new amides are reported from the seeds extract of Piper nigrum Linn. ► Their structures were elucidated on the basis of modern spectroscopic methods. ► The larvicidal activity was determined by WHO method. Six bioactive compounds were isolated from the seeds extract of Piper nigrum Linn. following a larvicidal activity guided isolation against 4th instar larvae of Aedes aegypti L., a Dengue vector mosquito and a carrier of yellow fever. Their structures were elucidated using spectroscopic methods including HR-EI-MS, FAB-MS, 1H and 13C NMR (Broad Bond Decoupled, & DEPT), and 2D-NMR techniques (1H–1H COSY, NOESY, HMQC, HMBC, & 2D-J-resolved). These include three new constituents namely pipilyasine (1), pipzubedine (2) and pipyaqubine (3), and three known constituents pellitorine (4), pipericine (5) and piperine (6). The larvicidal activity was determined by WHO method.