FLRT3 Is a Robo1-Interacting Protein that Determines Netrin-1 Attraction in Developing Axons

Guidance molecules are normally presented to cells in an overlapping fashion; however, little is known about how their signals are integrated to control the formation of neural circuits. In the thalamocortical system, the topographical sorting of distinct axonal subpopulations relies on the emergent cooperation between Slit1 and Netrin-1 guidance cues presented by intermediate cellular targets. However, the mechanism by which both cues interact to drive distinct axonal responses remains unknown. Here, we show that the attractive response to the guidance cue Netrin-1 is controlled by Slit/Robo1 signaling and by FLRT3, a novel coreceptor for Robo1. While thalamic axons lacking FLRT3 are insensitive to Netrin-1, thalamic axons containing FLRT3 can modulate their Netrin-1 responsiveness in a context-dependent manner. In the presence of Slit1, both Robo1 and FLRT3 receptors are required to induce Netrin-1 attraction by the upregulation of surface DCC through the activation of protein kinase A. Finally, the absence of FLRT3 produces defects in axon guidance in vivo. These results highlight a novel mechanism by which interactions between limited numbers of axon guidance cues can multiply the responses in developing axons, as required for proper axonal tract formation in the mammalian brain. [Display omitted] •Netrin-1 attraction requires DCC upregulation through Slit1/Robo1 signaling•FLRT3 is a modulator of Netrin-1 response•FLRT3 is a Robo1 coreceptor•Altering FLRT3 function affects thalamocortical axon pathfinding in vivo Leyva-Díaz et al. use different experimental approaches to understand how interactions between guidance cues can multiply axonal responses that are necessary for the formation of neural circuits. Specifically, Netrin-1 attraction in thalamic axons is controlled by Slit/Robo1 signaling and by FLRT3, a novel Robo1 coreceptor.