A role of zinc-finger protein 143 for cancer cell migration and invasion through ZEB1 and E-cadherin in colon cancer cells

To investigate the role of zinc‐finger protein 143 in cancer cells, we stably introduced ZNF143 expression knockdown by infecting colon cancer cells with short hairpin (sh) RNA‐lentiviral particles against ZNF143 (HCT116 sh‐ZNF143). Compared to sh‐control cells, HCT116 sh‐ZNF143 cells showed faster...

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Veröffentlicht in:	Molecular carcinogenesis 2014-02, Vol.53 (S1), p.E161-E168
Hauptverfasser:	Paek, A Rome, Lee, Chang-Hoon, You, Hye Jin
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis Blotting, Western Cadherins - genetics Cadherins - metabolism Cell Adhesion Cell Movement Cell Proliferation Colonic Neoplasms - genetics Colonic Neoplasms - metabolism Colonic Neoplasms - pathology E-cadherin Flow Cytometry Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Immunoenzyme Techniques invasion migration Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Small Interfering - genetics Signal Transduction Trans-Activators - physiology Transcription Factors - genetics Transcription Factors - metabolism Tumor Cells, Cultured Wound Healing ZEB1 Zinc Finger E-box-Binding Homeobox 1 ZNF143
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Zusammenfassung:	To investigate the role of zinc‐finger protein 143 in cancer cells, we stably introduced ZNF143 expression knockdown by infecting colon cancer cells with short hairpin (sh) RNA‐lentiviral particles against ZNF143 (HCT116 sh‐ZNF143). Compared to sh‐control cells, HCT116 sh‐ZNF143 cells showed faster wound healing, increased migration through Transwell chambers, and increased invasion through Matrigel in Transwell chambers. ZNF143 knockdown increased transcriptional expression of ZEB1. Additionally, ZNF143 regulated E‐cadherin transcriptional expression. Small interfering‐RNA‐mediated silencing of ZEB1 expression affected motility in HCT116 sh‐ZNF143 cells. These data suggest that ZNF143 is involved in cellular motility through a ZEB1‐E‐cadherin‐linked pathway in colon cancer cells. © 2013 Wiley Periodicals, Inc.
ISSN:	0899-1987 1098-2744
DOI:	10.1002/mc.22083