Use of heat shock protein mRNA expressions as biomarkers in wild crucian carp for monitoring water quality

Highlights • Parts of the HSP30, 60, 70, and 90 genes in crucian carp were isolated and sequenced. • Their mRNA expression levels in wild crucian carp from Hun River were detected. • Increasing of HSP70 mRNA levels in the liver and the HSP30 mRNA levels in kidney were found in fish from downstream o...

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Veröffentlicht in:	Environmental toxicology and pharmacology 2014-01, Vol.37 (1), p.248-255
Hauptverfasser:	An, Li-hui, Lei, Kun, Zheng, Bing-hui
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biomarker Biomarkers - metabolism Carps - genetics Crucian carp DNA, Complementary - genetics Emergency Environmental Monitoring - methods Heat shock protein Heat-Shock Proteins - genetics Kidney - metabolism Liver - metabolism Phylogeny Quantitative real-time reverse transcription PCR Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sequence Analysis, DNA Stressor Water Quality
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Beschreibung
Zusammenfassung:	Highlights • Parts of the HSP30, 60, 70, and 90 genes in crucian carp were isolated and sequenced. • Their mRNA expression levels in wild crucian carp from Hun River were detected. • Increasing of HSP70 mRNA levels in the liver and the HSP30 mRNA levels in kidney were found in fish from downstream of the Hun River. • HSPs expressions were correlated with the changing of water quality in the river.
ISSN:	1382-6689 1872-7077
DOI:	10.1016/j.etap.2013.11.019