Structure elucidation of the blood group B like and blood group I active octaantennary ceramide tetracontasaccharide from rabbit erythrocyte membranes by two-dimensional super(1)H NMR spectroscopy at 600 MHz

The primary structure of the ceramide tetracontasaccharide from rabbit erythrocyte membranes has been determined. It was shown that obtaining subspectra of the constituent sugar residues from a totally correlated spectrum and assigning the resonances occurring in these subspectra by analyzing the relevant cross-peaks in phase-sensitive correlated spectra is the most efficient way for establishing complex oligosaccharide structures.