Non-invasive delivery of nanoparticles to hair follicles: A perspective for transcutaneous immunization

Highlights ► We investigated transfollicular vaccination via intact skin using nanoparticles(NP). ► Polymeric NP from PLGA+- chitosan were loaded with ovalbumin (OVA). ► OVA/NP stimulated proliferation of CD4+ &/or CD8+ T-cells more than OVA/solution. ► NP improved (×2–3) follicular delivery of...

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Veröffentlicht in:	Vaccine 2013-07, Vol.31 (34), p.3442-3451
Hauptverfasser:	Mittal, Ankit, Raber, Anne S, Schaefer, Ulrich F, Weissmann, Sebastian, Ebensen, Thomas, Schulze, Kai, Guzmán, Carlos A, Lehr, Claus-Michael, Hansen, Steffi
Format:	Artikel
Sprache:	eng
Schlagworte:	Administration, Cutaneous Adoptive transfer Allergy and Immunology Animals antigen-presenting cells antigens Antigens - administration & dosage Antigens - immunology Applied microbiology bioactive properties biodegradability Biological activity Biological and medical sciences CD4 CD8 CD8-positive T-lymphocytes cell culture Cell Proliferation Chitosan Chitosan - administration & dosage Chitosan - immunology culture media Dendritic Cells - immunology Drug delivery ears Efficiency emulsions Experiments Female freeze drying Fundamental and applied biological sciences. Psychology Hair Follicle - immunology Hair Follicle - metabolism hair follicles immune response Immune system Immunization Immunization - methods Mice Mice, Inbred BALB C Mice, Inbred C57BL Microbiology Nanoparticle nanoparticles Nanoparticles - administration & dosage Nanoparticles - chemistry Older people Ovalbumin Ovalbumin - administration & dosage Ovalbumin - immunology PLGA Polyglactin 910 - chemistry Polyvinyl alcohol Studies Swine T-Lymphocytes - immunology Transcutaneous immunization Transfollicular immunization vaccination Vaccines Vaccines - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Beschreibung
Zusammenfassung:	Highlights ► We investigated transfollicular vaccination via intact skin using nanoparticles(NP). ► Polymeric NP from PLGA+- chitosan were loaded with ovalbumin (OVA). ► OVA/NP stimulated proliferation of CD4+ &/or CD8+ T-cells more than OVA/solution. ► NP improved (×2–3) follicular delivery of OVA on pig ears compared to OVA/solution. ► Consequently using OVA/NP one may reduce the dose compared to OVA/solution.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2012.12.048