Generation and characterization of a recombinant chimeric protein (rCpLi) consisting of B-cell epitopes of a dermonecrotic protein from Loxosceles intermedia spider venom

Highlights • A protein consisting of epitopes of a spider venom protein was engineered. • The chimeric protein generated did not show toxic activities. • Antibodies anti-chimeric protein recognized three different antigenic regions. • Antibodies anti-chimeric protein showed neutralizing potential. •...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Vaccine 2013-06, Vol.31 (25), p.2749-2755
Hauptverfasser:	Mendes, T.M, Oliveira, D, Figueiredo, L.F.M, Machado-de-Avila, R.A, Duarte, C.G, Dias-Lopes, C, Guimarães, G, Felicori, L, Minozzo, J.C, Chávez-Olortegui, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Accidents Allergy and Immunology amino acid sequences Animals Antibodies, Neutralizing - immunology Antivenins - immunology Applied microbiology Araneae B-cell epitopes B-lymphocytes Biological and medical sciences blood serum cellulose chimerism Dermonecrotic protein Edema - immunology Edema - prevention & control enzyme-linked immunosorbent assay epitopes Epitopes, B-Lymphocyte - genetics Fundamental and applied biological sciences. Psychology Hemorrhage - immunology Hemorrhage - prevention & control Loxosceles Loxosceles intermedia Loxosceles spider venom Microbiology Necrosis - immunology Necrosis - prevention & control neutralizing antibodies peptides Rabbits Recombinant chimeric protein Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Skin - immunology Skin - pathology Sphingomyelin Phosphodiesterase - chemistry Sphingomyelin Phosphodiesterase - genetics Sphingomyelin Phosphodiesterase - immunology Spider Venoms - chemistry Spider Venoms - genetics Spider Venoms - immunology Spiders vaccination vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) venoms
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Highlights • A protein consisting of epitopes of a spider venom protein was engineered. • The chimeric protein generated did not show toxic activities. • Antibodies anti-chimeric protein recognized three different antigenic regions. • Antibodies anti-chimeric protein showed neutralizing potential. • This protein is a candidate for therapeutic serum development.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2013.03.048