Butyric acid production from sugarcane bagasse hydrolysate by Clostridium tyrobutyricum immobilized in a fibrous-bed bioreactor

► We studied butyric acid production from sugarcane bagasse (SCB) hydrolysate. ► High sugar yields were obtained from SCB after acid pretreatment and enzymatic hydrolysis. ► C. tyrobutyricum was immobilized in a FBB for fed-batch fermentation of SCB hydrolysate. ► High butyric acid yield (>0.45g/...

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Veröffentlicht in:Bioresource technology 2013-02, Vol.129, p.553-560
Hauptverfasser: Wei, Dong, Liu, Xiaoguang, Yang, Shang-Tian
Format: Artikel
Sprache:eng
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Zusammenfassung:► We studied butyric acid production from sugarcane bagasse (SCB) hydrolysate. ► High sugar yields were obtained from SCB after acid pretreatment and enzymatic hydrolysis. ► C. tyrobutyricum was immobilized in a FBB for fed-batch fermentation of SCB hydrolysate. ► High butyric acid yield (>0.45g/gsugar) and productivity (0.51g/Lh) were obtained. ► This study demonstrated the feasibility of producing butyric acid from low-cost SCB feedstock. A fermentation process using Clostridium tyrobutyricum immobilized in a fibrous-bed bioreactor (FBB) was developed for butyric acid production from sugarcane bagasse (SCB) hydrolysate. SCB was first treated with dilute acid and then hydrolyzed with cellulases. The hydrolysate containing glucose and xylose was used as carbon source for the fermentation without detoxification. The bacterium was able to grow at a specific growth rate of ∼0.06h−1 in media containing 15–20% (w/v) SCB in serum bottles. In batch cultures in the FBB, both glucose and xylose in the SCB hydrolysate were simultaneously converted to butyrate with a high yield (0.45–0.54g/gsugar) and productivity (0.48–0.60g/Lh). A final butyrate concentration of 20.9g/L was obtained in a fed-batch culture, with an overall productivity of 0.51g/Lh and butyrate yield of 0.48g/g sugar consumed. This work demonstrated the feasibility of using SCB as a low-cost feedstock to produce butyric acid.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.11.065