Characterization of the alfalfa (Medicago sativa) genome by DNA reassociation

Total leaf DNA from diploid alfalfa, Medicago sativa HG-2 was purified by CsCl banding and treatment with RNAse A and proteinase K. Control DNA for reassociation analysis was Escherichia coli (K-12) DNA. The degree of reassociation was measured spectrophotometrically by following the decrease in hyp...

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Veröffentlicht in:	Plant molecular biology 1988-07, Vol.10 (4), p.369-371
Hauptverfasser:	WINICOV, I, MAKI, D. H, WATERBORD, J. H, RIEHM, M. R, HARRINGTON, R. E
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Biological and medical sciences Classical and quantitative genetics. Population genetics. Molecular genetics Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genes. Genome Genetics and breeding of economic plants Medicago sativa Molecular and cellular biology Molecular genetics
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Beschreibung
Zusammenfassung:	Total leaf DNA from diploid alfalfa, Medicago sativa HG-2 was purified by CsCl banding and treatment with RNAse A and proteinase K. Control DNA for reassociation analysis was Escherichia coli (K-12) DNA. The degree of reassociation was measured spectrophotometrically by following the decrease in hyperchromicity at 260 nm with DNA concentrations of 35-1245 mu g/ml. Reassociation of the different DNA classes showed 4-5% mismatch as determined by delta T sub(m) at the end of reassociation.
ISSN:	0167-4412 1573-5028
DOI:	10.1007/BF00029888