Blu-ray disk lens as the objective of a miniaturized two-photon fluorescence microscope

In this paper, we examine the performance of a Blu-ray disk (BD) aspheric lens as the objective of a miniaturized scanning nonlinear optical microscope. By combining a single 2D micro-electro mechanical system (MEMS) mirror as the scanner and with different tube lens pairs, the field of view (FOV) o...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Optics express 2013-12, Vol.21 (25), p.31604-31614
Hauptverfasser:	Chung, Hsiang-Yu, Kuo, Wei-Cheng, Cheng, Yu-Hsiang, Yu, Che-Hang, Chia, Shih-Hsuan, Lin, Cheng-Yung, Chen, Jie-Shin, Tsai, Huai-Jen, Fedotov, Andrey B, Ivanov, Anatoly A, Zheltikov, Aleksei M, Sun, Chi-Kuang
Format:	Artikel
Sprache:	eng
Schlagworte:	Compact Disks Equipment Design Equipment Failure Analysis Image Enhancement - instrumentation Lenses Microscopy, Fluorescence, Multiphoton - instrumentation Miniaturization
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	In this paper, we examine the performance of a Blu-ray disk (BD) aspheric lens as the objective of a miniaturized scanning nonlinear optical microscope. By combining a single 2D micro-electro mechanical system (MEMS) mirror as the scanner and with different tube lens pairs, the field of view (FOV) of the studied microscope varies from 59 μm × 93 μm up to 178 μm × 280 μm, while the corresponding lateral resolution varies from 0.6 μm to 2 μm for two-photon fluorescence (2PF) signals. With a 34/s video frame rate, in vivo dynamic observation of zebrafish heartbeat through 2PF of the excited green fluorescence protein (GFP) is demonstrated.
ISSN:	1094-4087 1094-4087
DOI:	10.1364/OE.21.031604