Direct imaging of fluorescent structures behind turbid layers
We present a method to directly image fluorescent structures inside turbid media. This is based on wave-front shaping to optimize the scattered light onto a single fluorescent particle, as the optical memory effect for a scanning image of the surroundings of this particle. We show that iterating the...
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Veröffentlicht in: | Optics express 2014-01, Vol.22 (2), p.1981-1989 |
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Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
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Online-Zugang: | Volltext |
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Zusammenfassung: | We present a method to directly image fluorescent structures inside turbid media. This is based on wave-front shaping to optimize the scattered light onto a single fluorescent particle, as the optical memory effect for a scanning image of the surroundings of this particle. We show that iterating the optimization leads to the focusing on a single particle whose surroundings are subsequently scanned. In combination with a parabolic phase pattern, this method can be extended to a three dimensional imaging method inside turbid media. |
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ISSN: | 1094-4087 1094-4087 |
DOI: | 10.1364/OE.22.001981 |