Direct imaging of fluorescent structures behind turbid layers

We present a method to directly image fluorescent structures inside turbid media. This is based on wave-front shaping to optimize the scattered light onto a single fluorescent particle, as the optical memory effect for a scanning image of the surroundings of this particle. We show that iterating the...

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Veröffentlicht in:Optics express 2014-01, Vol.22 (2), p.1981-1989
Hauptverfasser: Ghielmetti, Giulia, Aegerter, Christof M
Format: Artikel
Sprache:eng
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Zusammenfassung:We present a method to directly image fluorescent structures inside turbid media. This is based on wave-front shaping to optimize the scattered light onto a single fluorescent particle, as the optical memory effect for a scanning image of the surroundings of this particle. We show that iterating the optimization leads to the focusing on a single particle whose surroundings are subsequently scanned. In combination with a parabolic phase pattern, this method can be extended to a three dimensional imaging method inside turbid media.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.22.001981