Monoclonal antibodies to the molluscan small cardioactive peptide SCP sub(B): Immunolabeling of neurons in diverse invertebrates

The authors reported a development of murine monoclonal antibodies to a molluscan small cardioactive peptide (SCP sub(B)) and their application to immunolabeling of neurons in several molluscan and arthropod species. Monoclonal antibodies of the IgM class labeled identified SCP-containing neuron B11 in the frozen sections of the buccal ganglia of Tritonia diomedia). Neurons B11, B12, and GE1 and several other neurons of the buccal and gastroesophageal ganglia of T. diomedia bound the antibody, and a similar pattern of immunolabeling was found in the closely related gastropod Tritonia festiva . The authors also observed SCP sub(B)-like immunoreactivity in the central neurons of other nudibranch and pulmonate molluscs and in examples of insect (Acheta domesticus) and Thermobia domestica ) and crustacean (Semibalanus cariosus ) classes of the Arthropoda.