Cloning of Xanthomonas DNA that expresses d-xylose catabolic enzymes

Cloning of Xanthomonas DNA that expresses d-xylose catabolic enzymes

A gene bank of the d-xylose utilizing, cellulolytic Xanthomonas (XA1-1) DNA, inserted into the Hind III site of pKT230, was screened for clones which encoded d-xylose isomerase. One clone (pND70) was identified which complemented d-xylose isomerase negative mutants of Escherichia coli and this clone...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of biotechnology 1987, Vol.6 (2), p.159-165
Hauptverfasser: Liu, Chun-Qiang, Lucas, Rachel J., Daly, Jan M., Rickard, Pamela A.D., Dunn, Noel W.
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
Cloning
d-Xylose isomerase
Escherichia coli
Expression
Fundamental and applied biological sciences. Psychology
genes
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular cloning
Xanthomonas
Xanthomonas albilineans
xylose isomerase
xylose permease
xylulose kinase
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A gene bank of the d-xylose utilizing, cellulolytic Xanthomonas (XA1-1) DNA, inserted into the Hind III site of pKT230, was screened for clones which encoded d-xylose isomerase. One clone (pND70) was identified which complemented d-xylose isomerase negative mutants of Escherichia coli and this clone contained an insert of XA1-1 DNA of approximately 15 kb. Enzyme assays showed that pND70 appeared to encode d-xylose permease, and xylulose kinase in addition to d-xylose isomerase. Specific activities of all 3 enzymes from E. coli JA200 (pND70) grown in d-xylose were double those detected in XA1-1 when also grown on d-xylose.
ISSN:0168-1656
1873-4863
DOI:10.1016/0168-1656(87)90053-8