Role of complementary E sub(2) and D sub(2) sites in the reaction between fibrin and fibrinogen

The influence of fibrinogen on two stages of the process of polymerization of two forms of fibrin, differing in the set of polymerization sites (fibrin-desAA and fibrin-desAABB), was studied. It was concluded that the high sensitivity of fibrin-desAABB to fibrinogen is due to interaction of the active site E sub(2) with the site D sub(2) in the fibrinogen molecule. The concentration dependence of the inactivating effect of the tetrapeptide Gly-His-Arg-Pro on fibrinogen was investigated, along with the influence of temperature and Ca super(2+) ions on the interaction of the tetrapeptide with fibrinogen.