Immobilization of fungal peroxidase on alkylaminated organic and inorganic porous supports

Several alkylaminated porous silica gels and acrylic type porous polymers have been used for covalent binding of fungal peroxidase from Trametes versicolor. The immobilization efficiency expressed in terms of the bound protein content, specific enzyme activity, and enzyme storage stability have been...

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Veröffentlicht in:Acta biotechnologica 1988, Vol.8 (1), p.47-53
Hauptverfasser: Łobarzewski, J., Kolarz, B. N., Wojcik, A., Wojaczynska, M., Trochimczuk, A., Blaszczynska, T.
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Sprache:eng
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Zusammenfassung:Several alkylaminated porous silica gels and acrylic type porous polymers have been used for covalent binding of fungal peroxidase from Trametes versicolor. The immobilization efficiency expressed in terms of the bound protein content, specific enzyme activity, and enzyme storage stability have been determined for both types of supports used. The results indicate a better immobilization ability of organic polymers in comparison with silica gels.
ISSN:0138-4988
1521-3846
DOI:10.1002/abio.370080108