Preparation of polyclonal antibody highly specific for mouse BRD7 protein and its application

BRD7 was first identified as a novel bromodomain gene related to nasopharyngeal carcinoma （NPC） [1,2]. Over- expression of BRD7 in NPC cells inhibits cell growth and cell cycle progression from G1 to S phase by transcriptional regulation of important molecules involved in Ras/MEK/ ERK, Rb/E2F, and [3-catenin pathways [3-5]. BRD7 is mainly localized in the nucleus and possesses a functional nuclear localization signal sequence [5]. It has been identi- fied as a subunit of the SWI/SNF chromatin-remodeling complexes and is involved in the regulation of transcription as a transcription factor [6,7].