Internal initiation of polyuridylic acid translation in bacterial cell-free system

The task of the present work was to answer the question: is the free 5′-end needed for effective translation of a model polyribonucleotide template — polyuridylic acid — in a bacterial ( E. coli ) cell-free system? For this purpose, the template activities of the original polyuridylic acid with its...

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Veröffentlicht in:	Biochemistry (Moscow) 2013-12, Vol.78 (12), p.1354-1357
Hauptverfasser:	Sogorin, E. A., Agalarov, S. Ch, Spirin, A. S.
Format:	Artikel
Sprache:	eng
Schlagworte:	5' Flanking Region Acids Bacteriology Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Biosynthesis Cell-Free System Codon, Initiator E coli Enzymes Escherichia coli Escherichia coli - metabolism Fluorescein - chemistry Genetic aspects Genetic translation Life Sciences Microbiology Oligonucleotides - chemistry Physiological aspects Poly U - chemistry Poly U - metabolism Protein Biosynthesis Ribonucleases - metabolism Ribonucleic acid Ribosomes Ribosomes - metabolism RNA
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