Arteether Exerts Antitumor Activity and Reduces CD4 super( +)CD25 super( +)FOXP3 super( +) T-reg Cells in Vivo

Chemo-immunotherapy is one of the new achievements for treatment of cancer, by which the success of anti-cancer therapy can be increased. In vitro studies have been shown that Arteether (ARE) induces apoptosis in tumor cells, but not in normal cells. The objective of this study is to investigate the cytotoxic and immunomodulatory properties of Arteether in-vivo and in-vitro. In this study, the authors have used MTT assay for evaluation of cytotoxicity of Arteether on tumor cell line and Peripheral Blood Mononuclear Cells (PBMC) from healthy individuals. Balb/c mice were subcutaneously transplanted with tumor tissue taken from Spontaneous Mouse Mammary Tumor bearing female mice. They observed that ARE could reduce the cell growth of 4T1 cell line in a dose-dependent manner but it had no cytotoxic effect on the growth of peripheral blood lymphocytes. ARE administered intraperitoneally to tumor-bearing Balb/c mice could reduce the tumor growth rate and splenic T-reg cells. These results underscore antitumor properties of Arteether that may aid in development of more effective antitumor agents.