Novel procedure of elution and concentration of nucleic acids with NACS Prepac super(TM) minicolumns by electrophoresis

The innumerable procedures for the elution of nucleic acids from agarose gel have mainly two pitfalls: first, many subsequently intended enzyme reactions are interfered with by "inhibitory" constitutents of agarose, second, they are too time consuming if larger numbers of samples are to be handled. The authors were successful in avoiding these complications when preparing large numbers of different size class nucleic acid samples for UV-spectrophotometry, hybridization, transcription (with SP6 RNA-Polymerase), and DNA recombination in combining elution from agarose and concentration in NACS Prepac super(TM) minicolumns (BRL-Gibco) by electrophoresis.