Analysis of globin transition in Xenopus laevis and identification of globins by in vitro translation of hybrid-selected mRNA

The switch from larval to adult globin synthesis during amphibian metamorphosis was analyzed by polyacrylamide gel electrophoresis in acid urea-Triton X-100. 4–6 slowly migrating components were detected in larvae and at least 4 faster components appear at metamorphosis and persist in the adult stag...

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Veröffentlicht in:	FEBS letters 1986-09, Vol.205 (2), p.219-222
Hauptverfasser:	Sandmeier, E., Gygi, D., Wyler, T., Nyffenegger, U., Weber, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	(Xenopus laevis) Globin Electrophoresis Hybrid selection Translation (Xenopus laevis)GlobinElectrophoresisHybrid selectionTranslation Applied sciences Biological and medical sciences Exact sciences and technology Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Other techniques and industries Translation. Translation factors. Protein processing Xenopus laevis
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Zusammenfassung:	The switch from larval to adult globin synthesis during amphibian metamorphosis was analyzed by polyacrylamide gel electrophoresis in acid urea-Triton X-100. 4–6 slowly migrating components were detected in larvae and at least 4 faster components appear at metamorphosis and persist in the adult stage. To relate the globin subunits to the mRNA sequences, isolated as cDNA clones, mRNAs were hybrid-selected from total RNA of larval and adult erythrocytes and translated in a wheat germ system. The translation products were identified by comparison with the globin pattern of hemolysates
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(86)80901-2