C-ERC/mesothelin provokes lymphatic invasion of colorectal adenocarcinoma

Background Lymph node metastasis is a key event of colorectal cancer (CRC) progression. Mesothelin is expressed in various types of malignant tumor and associated with an unfavorable prognosis. The full-length mesothelin (Full-ERC) is cleaved by protease into membrane-bound C-ERC/mesothelin and N-ER...

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Veröffentlicht in:Journal of gastroenterology 2014, Vol.49 (1), p.81-92
Hauptverfasser: Kawamata, Futoshi, Homma, Shigenori, Kamachi, Hirofumi, Einama, Takahiro, Kato, Yasutaka, Tsuda, Masumi, Tanaka, Shinya, Maeda, Masahiro, Kajino, Kazunori, Hino, Okio, Takahashi, Norihiko, Kamiyama, Toshiya, Nishihara, Hiroshi, Taketomi, Akinobu, Todo, Satoru
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Sprache:eng
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Zusammenfassung:Background Lymph node metastasis is a key event of colorectal cancer (CRC) progression. Mesothelin is expressed in various types of malignant tumor and associated with an unfavorable prognosis. The full-length mesothelin (Full-ERC) is cleaved by protease into membrane-bound C-ERC/mesothelin and N-ERC/mesothelin which is secreted into the blood. The aim of this study was to examine the biological role of mesothelin in CRC by clinicopathological analysis and in vitro lymphatic invasion assay. Methods Ninety-one cases of CRC specimens were immunohistochemically examined and the localization of mesothelin in luminal membrane and/or cytoplasm was also evaluated. Lymphatic invasion assay was also performed using the human CRC cell line, WiDr, which was transfected with Full-, N- and C-ERC/mesothelin expression plasmids (Full-WiDr, N-WiDr and C-WiDr). Results Immunohistochemically, “luminal membrane positive” of mesothelin was identified in 37.4 %, and correlated with lymphatic permeation and lymph node metastasis, but not with patients’ prognosis. Interestingly, among the patients with lymph node metastasis ( N  = 38), “luminal membrane positive” of mesothelin significantly correlated with unfavorable patients’ outcome. In addition, lymphatic invasion assay revealed that Full-WiDr and C-WiDr more significantly invaded human lymphatic endothelial cells than the Mock-WiDr ( P  
ISSN:0944-1174
1435-5922
DOI:10.1007/s00535-013-0773-6