Molecular cloning and expression of hybridoma growth factor in Escherichia coli

Molecular cloning and expression of hybridoma growth factor in Escherichia coli

By using expression cloning in Escherichia coli of complementary DNA derived from human monocyte-poly(A super(+)) RNA, the authors selected seven clones producing HGF activity as measured in a bioassay, based on the induction of proliferation of the HGF-dependent B cell hybridoma B9. Sequence analys...

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Veröffentlicht in:The Journal of immunology (1950) 1987-01, Vol.139 (2), p.4116-4121
Hauptverfasser: Brakenhoff, JPJ, de Groot, ER, Evers, R F, Pannekoek, H, Aarden, LA
Format: Artikel
Sprache:eng
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Zusammenfassung:By using expression cloning in Escherichia coli of complementary DNA derived from human monocyte-poly(A super(+)) RNA, the authors selected seven clones producing HGF activity as measured in a bioassay, based on the induction of proliferation of the HGF-dependent B cell hybridoma B9. Sequence analysis of the cDNA revealed that HGF is identical with interferon- beta 2, 26,000 protein, and B cell stimulatory factor-2.
ISSN:0022-1767