Dehydroalanine-Based Diubiquitin Activity Probes

Dehydroalanine-Based Diubiquitin Activity Probes

A strategy for the synthesis of dehydroalanine based diubiquitin activity probes is described. The site-specific introduction of dehydroalanine was achieved from diubiquitin bearing Cys residue near the scissile bond between two ubiquitins linked through Lys48, Lys63 or in a head to tail fashion. Th...

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Veröffentlicht in:Organic letters 2014-01, Vol.16 (2), p.540-543
Hauptverfasser: Haj-Yahya, Najat, Hemantha, Hosahalli P, Meledin, Roman, Bondalapati, Somasekhar, Seenaiah, Mallikanti, Brik, Ashraf
Format: Artikel
Sprache:eng
Schlagworte:
Alanine - analogs & derivatives
Alanine - chemistry
Amino Acid Sequence
Humans
Lysine - chemistry
Models, Molecular
Molecular Structure
Protein Conformation
Ubiquitin-Specific Proteases - metabolism
Ubiquitins - metabolism
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Zusammenfassung:A strategy for the synthesis of dehydroalanine based diubiquitin activity probes is described. The site-specific introduction of dehydroalanine was achieved from diubiquitin bearing Cys residue near the scissile bond between two ubiquitins linked through Lys48, Lys63 or in a head to tail fashion. The probes were characterized for their activities with various deubiquitinases, which open new opportunities in studying deubiquitinases in various settings.
ISSN:1523-7060
1523-7052
DOI:10.1021/ol403416w