Preparation of carbon paste electrodes including poly(styrene) attached glycine–Pt(IV) for amperometric detection of glucose

In this study, a novel carbon paste electrode that is sensitive to glucose was prepared using the nanoparticles modified (4-Formyl-3-methoxyphenoxymethyl) with polystyren (FMPS) with l-Glycine–Pt(IV) complexes. Polymeric nanoparticles having Pt(IV) ion were prepared from (4-Formyl-3-methoxyphenoxymethyl) polystyren, glycine and PtCl4 by template method. Glucose oxidase enzyme was immobilized to a modified carbon paste electrode (MCPE) by cross-linking with glutaraldehyde. Determination of glucose was carried out by oxidation of enzymatically produced H2O2 at 0.5V vs. Ag/AgCl. Effects of pH and temperature were investigated, and optimum parameters were found to be 8.0 and 55°C, respectively. Linear working range of the electrode was 5.0×10−6–1.0×10−3M, R2=0.997. Storage stability and operational stability of the enzyme electrode were also studied. Glucose biosensor gave perfect reproducible results after 10 measurements with 2.3% relative standard deviation. Also, it had good storage stability (gave 53.57% of the initial amperometric response at the end of 33th day). •Novel nanoparticles modified (4-Formyl-3-methoxyphenoxy methyl) with polystyrene (FMPS) with l-Glycine–Pt(IV) complexes were synthesized.•A novel carbon paste electrode was prepared using the modified nanoparticles.•Novel nanoparticles provide a good environment for stable and reproducible immobilization of glucose oxidase.•Determination of glucose was carried out by the oxidation of enzymatically produced hydrogen peroxide.•The glucose biosensor characteristics (sensitivity, concentration range, reproducibility, pH, temperature and long-term stability) for biocatalytic oxidation of glucose can be improved significantly by using modified nanoparticles.•Glucose biosensor prepared in this study is easy to prepare and highly cost effective.