Multiple enzymatic profiles of Vibrio parahaemolyticus strains isolated from oysters
The enzymatic characterization of vibrios has been used as a virulence indicator of sanitary interest. The objective of this study was to determine the enzymatic profile of Vibrio parahaemolyticus strains (n=70) isolated from Crassostrea rhizophorae oysters. The strains were examined for the presenc...
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Veröffentlicht in: | Revista argentina de microbiología 2013-10, Vol.45 (4), p.267-270 |
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Zusammenfassung: | The enzymatic characterization of vibrios has been used as a virulence indicator of sanitary interest. The objective of this study was to determine the enzymatic profile of Vibrio parahaemolyticus strains (n=70) isolated from Crassostrea rhizophorae oysters. The strains were examined for the presence of gelatinase (GEL), caseinase (CAS), elastase (ELAS), phospholipase (PHOS), lipase (LIP), amilase (AML) and DNase. All enzymes, except elastase, were detected in more than 60% of the strains. The most recurrent enzymatic profiles were AML + DNase + PHOS + GEL + LIP (n=16; 22.9%) and AML + CAS + DNase + PHOS + GEL + LIP (n=21; 30%). Considering the fact that exoenzyme production by vibrios is closely related to virulence, one must be aware of the bacteriological risk posed to human health by the consumption of raw or undercooked oysters.
La caracterización enzimática de los vibrios se ha utilizado como un indicador de virulencia de interés sanitario. El objetivo de este estudio fue determinar el perfil enzimático de 70 cepas de Vibrio parahaemolyticus aisladas a partir de ostras Crassostrea rhizophorae. Se investigó la presencia de gelatinasa (GEL), caseinasa (CAS), elastasa (ELAS), fosfolipasa (PHOS), lipasa (LIP), amilasa (AML) y DNasa. Todas las enzimas se detectaron en más de 60% de las cepas, excepto la elastasa. Los perfiles enzimáticos más recurrentes fueron AML + DNasa + PHOS + GEL + LIP (n=16; 22,9%) y AML + CAS + DNasa + PHOS + GEL + LIP (n=21; 30%). Teniendo en cuenta el hecho de que la producción de exoenzimas por vibrios está estrechamente relacionada con la virulencia, se alerta acerca del riesgo bacteriológico para la salud humana asociado al consumo de ostras crudas o poco cocidas. |
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ISSN: | 0325-7541 1851-7617 |
DOI: | 10.1016/S0325-7541(13)70035-X |