Evidence for the Presence of Proteinase Inhibitor I in Vacuolar Protein Bodies of Plant Cells

Protein bodies induced in tomato leaf cells by wounding were shown to contain proteinase Inhibitor I by using ferritin-labelled antibodies, fluorescein-labelled antibodies, and cytochrome C-labelled antibody fragments. Both pre-embedding and post-embedding techniques were used. Nonspecific binding was least when p-formaldehyde was used as the initial fixative followed by treatment with cytochrome c-labelled antibody fragments.