Influence of manganese on apoptosis and glutathione content of cumulus cells during in vitro maturation in bovine oocytes

Influence of manganese on apoptosis and glutathione content of cumulus cells during in vitro maturation in bovine oocytes

We have investigated the effect of different Mn concentrations on (1) DNA integrity of cumulus cells by olive tail moment (OTM); (2) cumulus cells apoptosis by Annexin V staining assay; (3) intracellular total glutathione (GSH‐GSSG) content; and (4) oocyte nuclear maturation and embryo cleavage afte...

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Veröffentlicht in:Cell biology international 2014-02, Vol.38 (2), p.246-253
Hauptverfasser: Anchordoquy, Juan Patricio, Anchordoquy, Juan Mateo, Picco, Sebastián J., Sirini, Matías A., Errecalde, Ana Lía, Furnus, Cecilia C.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Apoptosis
Cattle
Cumulus Cells - cytology
Cumulus Cells - metabolism
Female
Fertilization in Vitro
follicular fluid
glutathione
Glutathione - metabolism
in vitro fertilisation
manganese
Manganese - analysis
Manganese - blood
Manganese - metabolism
oocyte maturation
Oocytes - cytology
Oocytes - metabolism
Oogenesis
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Zusammenfassung:We have investigated the effect of different Mn concentrations on (1) DNA integrity of cumulus cells by olive tail moment (OTM); (2) cumulus cells apoptosis by Annexin V staining assay; (3) intracellular total glutathione (GSH‐GSSG) content; and (4) oocyte nuclear maturation and embryo cleavage after in vitro fertilisation (IVF). For this purpose, 0 (control), 2 (Mn1), 5 (Mn2) and 6 ng/mL (Mn3) Mn concentrations were added to IVM medium. Comet assay analysed by OTM was significantly higher in cumulus cells arising from COCs matured without Mn (control, P 
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.10195