Bio-inspired encapsulation and functionalization of living cells with artificial shells

This tutorial review describes the development of novel methods for encapsulation of biological cells with polymers and nanomaterials, focusing on the areas of potential applications of functionalized cells. •The latest methods to fabricate encapsulated cells were discussed.•Examples of different types of artificial shells were listed.•Their advantages and disadvantages were compared.•The biomedical applications of encapsulated cells were also described. In nature, most single cells do not have structured shells to provide extensive protection apart from diatoms and radiolarians. Fabrication of biomimetic structures based on living cells encapsulated with artificial shells has a great impact on the area of cell-based sensors and devices as well as fundamental studies in cell biology. The past decade has witnessed a rapid increase of research concerning the new fabrication strategies, functionalization and applications of this kind of encapsulated cells. In this review, the latest fabrication strategies on how to encapsulate living cells with functional shells based on the diversity of artificial shells are discussed: hydrogel matrix shells, sol-gel shells, polymeric shells, and induced mineral shells. Classical different types of artificial shells are introduced and their advantages and disadvantages are compared and explained. The biomedical applications of encapsulated cells with particular emphasis on cell implant protection, cell separation, biosensors, cell therapy and tissue engineering are also described and a recap of this review and the future perspectives on these active areas is given finally.