Microflora and volatile fatty acids present in inguinal pouches of the wild rabbit,Oryctolagus cuniculus, in Australia

Contents of inguinal pouches of the wild rabbit,Oryctolagus cuniculus, have been examined for the presence of microorganisms and volatile fatty acids. A total of 245 isolates were made from 167 samples taken from 75 rabbits, and microorganisms were obtained from 93% of individuals. The most common microorganism encountered wasStaphylococcus aureus, followed by the yeastCandida kruzei. Other organisms isolated wereBacillus subtilis,Escherichia coli, andStreptococcus faecalis. Quantitative analysis of volatile fatty acids in scrapings from pouches showed considerable variation between individuals. Of the acids identified, acetic acid and isovaleric acid were the most abundant. In a liquid medium containing one of these acids, the growth of microorganisms was only slightly affected. However, growth became inhibited when these fatty acids were used in combination.