Isolation of Two Protein Factors from Maize Involved in Poly U-dependent Polyphenylalanine Synthesis by Maize Ribosomes

Isolation of Two Protein Factors from Maize Involved in Poly U-dependent Polyphenylalanine Synthesis by Maize Ribosomes

Two protein factors, isolated from S-100 of maize shoots, were found to be required not only for a "Mg2+ shift" for poly U-dependent polymerization of phenylalanine, but also for polyphenylalanine synthesis. They seem to be crude preparations of EF-1 and EF-2. The poly U-directed amino aci...

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Veröffentlicht in:Planta 1976-01, Vol.131 (1), p.47-50
Hauptverfasser: Sim, Woong-Seop, Klämbt, Dieter
Format: Artikel
Sprache:eng
Schlagworte:
Amino acids
Corn
Cytokinins
Peptide elongation factors
Polymerization
Protein synthesis
Proteins
Ribosomes
RNA
Transfer RNA
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Zusammenfassung:Two protein factors, isolated from S-100 of maize shoots, were found to be required not only for a "Mg2+ shift" for poly U-dependent polymerization of phenylalanine, but also for polyphenylalanine synthesis. They seem to be crude preparations of EF-1 and EF-2. The poly U-directed amino acid incorporation by washed ribosomes was completely dependent upon these factors.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00387344