Crosslinking of elongation factor Tu to tRNA super(Phe) by transdiammine-dichloroplatinum (II). Characterization of two crosslinking sites in the tRNA

Crosslinking of elongation factor Tu to tRNA super(Phe) by transdiammine-dichloroplatinum (II). Characterization of two crosslinking sites in the tRNA

Transdiammine-dichloroplatinum (II) was used to induce reversible crosslinks between EF-Tu and Phe-tRNA super(Phe) within the ternary EF-Tu/GTP/Phe-tRNA super(Phe) complex. Up to 40% of the complex was specifically converted into crosslinked species. Two crosslinking sites have been unambiguously id...

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Veröffentlicht in:Nucleic acids research 1987-01, Vol.15 (14), p.5787-5801
Hauptverfasser: Wikman, F P, Romby, P, Metz, M H, Reinbolt, J, Clark, BFC, Ebel, J P, Ehresmann, C, Ehresmann, B
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Sprache:eng
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Zusammenfassung:Transdiammine-dichloroplatinum (II) was used to induce reversible crosslinks between EF-Tu and Phe-tRNA super(Phe) within the ternary EF-Tu/GTP/Phe-tRNA super(Phe) complex. Up to 40% of the complex was specifically converted into crosslinked species. Two crosslinking sites have been unambiguously identified. The major one encompassing nucleotides 58 to 65 is located in the 3'-part of the T-stem, and the minor one encompassing nucleotides 31 to 42 includes the anticodon loop and part of the of 3'-strand of the anticodon stem.
ISSN:0305-1048