Localization of chemically induced chromosomal aberrations in three different karyotypes of Vicia faba (mitomycin, maleic hydrazide, hydroxyurea, 8-ethoxycaffeine)

For the purpose of mapping the distribution of chromosomal aberrations. Rieger and Michaelis have divided the chromosomes of Vicia faba into segments, numbered 1-28. The authors have used this mapping system in a study of the localization of aberrations in segments nos. 2 (= nucleolar constriction) and 4 after treatments of root tips of Vicia faba with mitomycin C (MMC), maleic hydrazide (MH), hydroxyurea (HU) and 8-ethoxy-caffeine (EOC). Seedlings of three different karyotypes were used in these experiments. In all three karyotype, the chromatid aberrations induced by MMC and MH were preferentially localized in segment 4, whereas a high frequency of theose induced by HU and EOC were localized in segment 2.