Comparison of two anti-T-2 toxin immunoglobulins by direct ELISA
Monoclonal anti-T-2 IgGs produced from 12C12 and 15H6 hybridomas were compared by enzyme-linked immunosorbent assay. Binding activity was linear from 0.005 to 0.25 mu g protein/ml with 15H6. The quantity of T-2 toxin ( mu g protein/ml) required for one-half maximum binding activity of 15H6 (0.0875 m...
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| Veröffentlicht in: | Biotechnology techniques 1988-03, Vol.2 (1), p.69-72 |
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| container_title | Biotechnology techniques |
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| creator | Rivers, D.B Frazer, F.R. III |
| description | Monoclonal anti-T-2 IgGs produced from 12C12 and 15H6 hybridomas were compared by enzyme-linked immunosorbent assay. Binding activity was linear from 0.005 to 0.25 mu g protein/ml with 15H6. The quantity of T-2 toxin ( mu g protein/ml) required for one-half maximum binding activity of 15H6 (0.0875 mu g protein/ml) was approximately 68% that of 12C12 ( mu g protein/ml). |
| doi_str_mv | 10.1007/BF01874211 |
| format | Article |
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III</creator><creatorcontrib>Rivers, D.B ; Frazer, F.R. III</creatorcontrib><description>Monoclonal anti-T-2 IgGs produced from 12C12 and 15H6 hybridomas were compared by enzyme-linked immunosorbent assay. Binding activity was linear from 0.005 to 0.25 mu g protein/ml with 15H6. The quantity of T-2 toxin ( mu g protein/ml) required for one-half maximum binding activity of 15H6 (0.0875 mu g protein/ml) was approximately 68% that of 12C12 ( mu g protein/ml).</description><identifier>ISSN: 0951-208X</identifier><identifier>EISSN: 1573-6784</identifier><identifier>DOI: 10.1007/BF01874211</identifier><identifier>CODEN: BTECE6</identifier><language>eng</language><publisher>London: Chapman and Hall</publisher><subject>aflatoxins ; Biological and medical sciences ; Biotechnology ; comparisons ; enzyme-linked immunosorbent assay ; Fundamental and applied biological sciences. Psychology ; Fusarium ; Health. 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Psychology</topic><topic>Fusarium</topic><topic>Health. Pharmaceutical industry</topic><topic>hybridomas</topic><topic>immunoglobulin G</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>mice</topic><topic>Miscellaneous</topic><topic>Monoclonal antibodies</topic><topic>Production of active biomolecules</topic><topic>T-2 toxin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rivers, D.B</creatorcontrib><creatorcontrib>Frazer, F.R. 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| fulltext | fulltext |
| identifier | ISSN: 0951-208X |
| ispartof | Biotechnology techniques, 1988-03, Vol.2 (1), p.69-72 |
| issn | 0951-208X 1573-6784 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14833743 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | aflatoxins Biological and medical sciences Biotechnology comparisons enzyme-linked immunosorbent assay Fundamental and applied biological sciences. Psychology Fusarium Health. Pharmaceutical industry hybridomas immunoglobulin G Industrial applications and implications. Economical aspects mice Miscellaneous Monoclonal antibodies Production of active biomolecules T-2 toxin |
| title | Comparison of two anti-T-2 toxin immunoglobulins by direct ELISA |
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