Comparison of two anti-T-2 toxin immunoglobulins by direct ELISA

Monoclonal anti-T-2 IgGs produced from 12C12 and 15H6 hybridomas were compared by enzyme-linked immunosorbent assay. Binding activity was linear from 0.005 to 0.25 mu g protein/ml with 15H6. The quantity of T-2 toxin ( mu g protein/ml) required for one-half maximum binding activity of 15H6 (0.0875 m...

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Veröffentlicht in:	Biotechnology techniques 1988-03, Vol.2 (1), p.69-72
Hauptverfasser:	Rivers, D.B, Frazer, F.R. III
Format:	Artikel
Sprache:	eng
Schlagworte:	aflatoxins Biological and medical sciences Biotechnology comparisons enzyme-linked immunosorbent assay Fundamental and applied biological sciences. Psychology Fusarium Health. Pharmaceutical industry hybridomas immunoglobulin G Industrial applications and implications. Economical aspects mice Miscellaneous Monoclonal antibodies Production of active biomolecules T-2 toxin
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Zusammenfassung:	Monoclonal anti-T-2 IgGs produced from 12C12 and 15H6 hybridomas were compared by enzyme-linked immunosorbent assay. Binding activity was linear from 0.005 to 0.25 mu g protein/ml with 15H6. The quantity of T-2 toxin ( mu g protein/ml) required for one-half maximum binding activity of 15H6 (0.0875 mu g protein/ml) was approximately 68% that of 12C12 ( mu g protein/ml).
ISSN:	0951-208X 1573-6784
DOI:	10.1007/BF01874211