Nucleotide sequence and linkage map position of the gene for maize chloroplast ribosomal protein S14

A cloned fragment of maize chloroplast DNA is shown to contain the nucleotide sequence that encodes the protein homologous to Escherichia coli ribosomal protein S14. The nucleotide sequence was determined by the dideoxy chain termination method using the M13mp phage system. The deduced protein sequence is 103 amino acids long and has 39% identity with the ribosomal protein S14 of E. coli and 76% identity with the S14 of Marchantia polymorpha chloroplast. The three homologous proteins exhibit a much closer identity at the level of predicted secondary structure.