Factors governing lactic acid formation in long term cultivation of hybridoma cells

Effect of glucose concentration and pH on lactic acid formation was investigated in batch cultures of a hybridoma cell line. Lactate formation increases with growth rate. High glucose concentration leads to extensive lactate formation only during growth phase and not during stationary phase. Lactate...

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Veröffentlicht in:	Biotechnology letters 1987-11, Vol.9 (11), p.751-756
Hauptverfasser:	LUAN, Y. T, MUTHARASAN, R, MAGEE, W. E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells batch culture Biological and medical sciences Biotechnology Cell hybridization, cell fusion, hybridomas Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology hybridoma lactic acid Methods. Procedures. Technologies
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Zusammenfassung:	Effect of glucose concentration and pH on lactic acid formation was investigated in batch cultures of a hybridoma cell line. Lactate formation increases with growth rate. High glucose concentration leads to extensive lactate formation only during growth phase and not during stationary phase. Lactate formation also may serve to regulate extracellular pH to pH 6.8, provided conditions are favorable to maintain viability and if sufficient nutrients are present in the medium.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/BF01028278