Molecular cloning of pea mRNAs encoding a shoot-specific polypeptide and light-induced polypeptides

The molecular cloning of cDNA corresponds to pea seedling mRNA sequences encoding a shoot-specific polypeptide, the small subunit of the ribulose 1,5 biphosphate carboxylase and a component of the light-harvesting chlorophyll a/b complex is described. cDNA prepared from polysomal poly(A)RNA of light...

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Veröffentlicht in:Plant molecular biology 1983-11, Vol.2 (6), p.295-303
Hauptverfasser: DE VRIES, S. C, HARMSEN, M. C, KUIPER, M. T. R, DONS, H. J. M, WESSELS, J. G. H
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Sprache:eng
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Zusammenfassung:The molecular cloning of cDNA corresponds to pea seedling mRNA sequences encoding a shoot-specific polypeptide, the small subunit of the ribulose 1,5 biphosphate carboxylase and a component of the light-harvesting chlorophyll a/b complex is described. cDNA prepared from polysomal poly(A)RNA of light-grown shoots was enriched for shoot-specific and light-induced sequences by heterologous liquid hybridization with mercurated polysomal poly(A)RNA of dark-grown roots, followed by sulfhydryl chromatography. Cloned shoot-specific sequences were identified by 2D electrophoretic analysis of hybrid release translation products. The cloned shoot-specific sequence corresponded to a mRNA of 850 nt present both in light-and dark-grown shoots, and produced anin vitro translation product of Mr27 500 and isoelectric point of 4.7.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF01578590